Biopolym. Cell. 2025; 41(4):275.
http://dx.doi.org/10.7124/bc.000B27
Molecular Biomedicine
Establishment of U-251 MG cell sublines with ectopic overexpression of CHI3L1 mRNA variants as a model system of the isoforms trials in gliomagenesis
1, 2Areshkov P. O., 1Anopriienko O. V., 1Shloma A. R., 1Solomiana K. I., 1, 2Zhuk O. V., 1, 2Bukreieva T. V., 2, 3, 4Rybachuk O. A., 3Nesterenko Yu. A., 1, 2Skrypkina I. Ya.
  1. Institute of Molecular Biology and Genetics, NAS of Ukraine
    150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03143
  2. Institute of Cell Therapy
    3, Liubomyra Huzara Ave., Kyiv, Ukraine, 03126
  3. O.O. Bogomoletz Institute of Physiology, NAS of Ukraine
    4, Akademika Bohomoltsia Str., Kyiv, Ukraine, 01024
  4. Institute of Genetic and Regenerative Medicine,
    M.D. Strazhesko National Scientific Center of Cardiology,
    Clinical and Regenerative Medicine, NAMS of Ukraine
    5, Sviatoslava Khorobroho Str., Kyiv, Ukraine, 03151

Abstract

Aim. Obtaining glioma derived U-251 MG cell sublines producing the full-length and the short variants of CHI3L1 with subsequent characterization. Methods. Cell lentiviral transduction, Western blot and RT-qPCR analysis. Results. We successfully generated the U-251 MG cell sublines producing the full-length and the short variants of CHI3L1; our results support the suppression of CHI3L1-del8ex secretion and demonstrate differential targeting of microenvironment (LOX and GUSB) and EMT program (TGFB1) modulators by CHI3L1 isoforms. Conclusion. Here we present the cell model for CHI3L1 isoforms trials in gliomagenesis regarding experiments on co-cultivation and/with 3D culturing.
Keywords: CHI3L1, glioblastoma, transcriptional isoforms, splicing, differentially expressing genes (DEG), epithelial-mesenchymal transition (EMT)
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